New Technologies, New Vaccines 2016

Poster Titles

Assessing the Tumorigenic Phenotypes of MDCK Populations
Romelda Omeir, Kathryn Phy, Wei Tu, Gideon Foseh, Andrew M. Lewis, Jr., and Keith Peden, Division of Viral Products, Office of Vaccines Research and Review, CBER, FDA, Silver Spring, MD 20993

Can Human Monoclonal Antibodies Contribute to the Global Polio Eradication Initiative?
Rama Devudu Puligedda1, Diana Kouiavskaia2, Chandana Devi Kattala1, Hamid Yaqoob1, Konstantin Chumakov2, and Scott K. Dessain1
1: Lankenau Institute for Medical Research, 100 E. Lancaster Ave, Wynnewood, PA 19096, USA
2: Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, MD 20852, USA

Cancer cells as “infectious agents” in the tumor microenvironment
Raj Rajasekaran, Ph.D. and Pratima Patil, Ph.D., Therapy Architects, LLC, Helen F. Graham Cancer Center, Newark, DE, and University of Delaware, Newark, DE

Cold Chain and Virus Free Plant-made Booster Vaccine to Confer Immunity Against Different Polio Virus Serotypes
Hui-Ting Chan1, Yuhong Xiao1, William C. Weldon2, Steven M. Oberste2, Konstantin Chumakov3, Henry Daniell1
1: Department of Biochemistry, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
2: Centers for Disease Control and Prevention, 1600 Clifton Rd NE, Mailstop G-17, Atlanta, GA 30329 USA
3: Center for Biologics Evaluation and Research, Food and Drug Administration Bldg. 29B, Rm. 5NN01, HFM-20 8800 Rockville Pike, Bethesda, MD 20892, USA.

Comparative study of native and codon optimized human and viral genes using genomic, proteomic and ribosome profiling enhances understanding of transgene expression in chloroplasts
Kwang-Chul Kwon1, Hui-Ting Chan1, Ileana R. León2, Rosalind Williams-Carrier3, Alice Barkan3, and Henry Daniell1
1: Departments of Biochemistry, School of Dental Medicine, University of Pennsylvania Philadelphia, PA 19104-6030, USA
2: Global Research, Novo Nordisk A/S, Måløv, DK-2760, Denmark
3: Institute of Molecular Biology, University of Oregon, Eugene, OR 97403-1229

Comprehensive Mutagenesis of Dengue Virus Envelope Proteins to Map Antibody Epitopes and Identify Resides Essential for Function.
Benjamin J. Doranz, Integral Molecular, Philadelphia, Pennsylvania

Correlation between in vivo immunogenicity and the thermal stability of a plant-produced yellow fever subunit vaccine
Huaxin Si1, Patricia C. C. Neves2, Jessica Chichester1, Slobodanka D. Manceva1, Mark Jones1, Stephen Streatfeild1, and Vidadi Yusibov1
1: Fraunhofer USA Center for Molecular Biotechnology
2: Bio-Maguinhos, Fiocruz, Brazil

Development of a Common Platform to Assess Neutralizing Antibodies to Pathogenic Human Viruses
Stella Lee, Kathryn Phy, Keith Peden, and Li Sheng-Fowler, Division of Viral Products, Office of Vaccines Research and Review, CBER, FDA, Silver Spring, MD 20993

Development of a Reference Virus Database (rVDB) for Enhancing Next-Generation Sequencing Analysis of Biologicals
Norman Goodacre1, Aisha Aljanahi1,3, Subhiksha Nandakumar1, J. Rodney Brister2, Arifa S. Khan1
1: Laboratory of Retroviruses, Division of Viral Products, Office of Vaccines Research and Review, CBER, U.S. Food and Drug Administration, Silver Spring, MD
2: National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD
3: Current Address: Georgetown University, Washington D.C.

Development of a two-step, cGMP-compliant Process for Adenovirus Purification
Mark A. Snyder1, Mark I. Fitchmun2* and John Chicca3
1: Process Chromatography Division, Bio-Rad Laboratories, Hercules CA United States
2: Somatek, Inc., San Diego, CA United States
3: Molecular Diagnostic Systems, Inc., San Diego, CA United States
*: Corresponding author. Phone +1-858-449-1310 E-mail:

Epigenetic Modulation Inhibits T-Regulatory Cell Mediated Cd8+ T Cell Dysfunction in Lentiviral Infection.
Mukta Nag, Yan Wang, Joanne Tuohy, Ravyn Njagu, Laura Edwards, Dan Bogan, Sarah Schuett, Jonathan Fogle, Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, U.S.A.

Epitope presentation of consensus dengue viral envelope glycoprotein domain III on plant-derived virus-like particles
Ee Leen Pang1, Hadrien Peyret2, Alex Ramirez3, Mike Whelan3, George P. Lomonossoff2, Kok-Song Lai4, and Sandy Hwei-San Loh1*
1: School of Biosciences, Faculty of Science, University of Nottingham Malaysia Campus, Selangor, Malaysia.
2: Department of Biological Chemistry, John Innes Centre, Norwich, United Kingdom.
3: iQur Ltd, London, United Kingdom.
4: Agro-Biotechnology Institute Malaysia, Selangor, Malaysia.
*: Corresponding author, Email:

Exposure of Epitope Residues on the Outer Face of the Chikungunya Virus Envelope Trimer Determines Antibody Neutralizing Efficacy.
Rachel H. Fong1, Soma S. R. Banik1, Jin Jing2, Graham Simmons2, and Benjamin J. Doranz1
1: Integral Molecular, Philadelphia, Pennsylvania
2: Blood Systems Research Institute, San Francisco, California

The Expression of Apoptin (Viral Protein 3) in Nicotiana benthamiana and its Bioactivity in Human Cancer Cells
Xiao-Ying Chan1,2, Sandy Hwei-San Loh1,2, Konstantin Musiychuk3, Yoko Shoji3, Mohd Azmi Mohd Lila4, and Vidadi Yusibov3
1: School of Biosciences, Faculty of Science
2: Biotechnology Research Centre, University of Nottingham Malaysia Campus, 43500 Semenyih, Malaysia
3: Fraunhofer USA Center for Molecular Biotechnology, 9 Innovation Way, Suite 200, Newark, DE, 19711, USA
4: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Malaysia

Formulation and In Vivo Immune Responses to Self-Emulsifying Lipid-Based Vaccine Adjuvants
Ruchi Shah1, Luis Brito2, Mansoor Amiji1 and Derek O’Hagan3
1: Northeastern University, Boston, MA, 02215
2: Moderna Therapeutics, Cambridge, MA, 02141
3: GlaxoSmithKline Vaccines, Cambridge, MA, 02139

In vitro Production, FPLC Purification and Functional Evaluation of an H5N1-HA Replicon RNA
Matthias Koch, Ph.D. and George W. Buchman, Ph.D., Paragon Bioservices 801 West Baltimore St., Suite 401, Baltimore, MD 21201

In Vivo Delivery of Live Attenuated Chikungunya Vaccine using DNA Vaccination
Peter Pushko, Medigen, Inc., Frederick, MD, USA

Madin Darby Canine Kidney Cell Single-Cell Clones Have an Unstable Non-tumorigenic Phenotype
Romelda Omeir, Wei Tu, Kathryn Phy, Gideon Foseh, Andrew M. Lewis, Jr., and Keith Peden, FDA

Magnetic Tunnel Junction Based Sensors for Bio Detection Applications
Harsha Kannan1, Aruna Sigdel1, Moneim Shamloul2, Stephen Streatfield2, Vidadi Yusibov2 & John Q. Xiao1
1: University of Delaware
2: Fraunhofer, USA

Manufacturing Drug Delivery Liposomes and Post Process Filtration
K. Jandrasitz1, Y. Su1, M. Folmsbee2, R. Turmell2, K. Marino2, B. Ayling1, and S. Mesite1
1: Microfluidics International Corporation, 90 Glacier Dr. Suite 1000, Westwood, MA 02090-1818, USA
2: Pall Life Sciences, 25 Harbor Park Dr, Port Washington, NY 11050, USA

Metabolic engineering of the vaccine adjuvant squalene via nuclear or chloroplast genomes profoundly and globally impacts off-target pathways through metabolite signaling
Elise K. Pasoreck1, Jin Su1, Ian M. Silverman2, Sager J. Gosai2, Brian D. Gregory2, Joshua S. Yuan3, Henry Daniell1
1: Department of Biochemistry, School of Dental Medicine
2: Department of Biology, University of Pennsylvania, Philadelphia, PA, 19104, USA
3: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, 77843, USA

Microbial Mining for the Identification of Novel Biomolecules
Aytan Aghayeva, Stephen Krasucki and Charles M. Morris, Fraunhofer USA

RT-qPCR-Based Microneutralization Assay for Human Cytomegalovirus Using Fibroblasts and Epithelial Cells
Xiao Wang, Keith Peden, and Haruhiko Murata, Division of Viral Products, Office of Vaccines Research and Review, CBER, FDA, Silver Spring, MD 20993

Suppression of antibody formation against human FIX in male hemophilia B dog and dosage studies in mice
Jin Su1, Alexandra Sherman2, Roland W. Herzog2, Timothy C. Nichols3, Henry Daniell1
1: Department of Biochemistry, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA
2: Department of Pediatrics, College of Medicine, University of Florida, Gainesville, FL, USA
3: Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

Two technologies for emerging threat response: DNA-launched and recombinant VLP vaccines
Peter Pushko, Medigen, Inc., 8420 Gas House Pike, Suite S, Frederick, MD 21701

Validation of the Potential miRNA Biomarkers for Detecting VERO-Cell Tumorigenicity Using a New Line (AGMK1-9T7) of African Green Monkey Kidney Cells
Belete Teferedegne, Daniel Rotroff, Juliete Macauley, Gideon Foseh, Gladys Lewis, Alison Motsinger, and Andrew M. Lewis Jr., DVP, OVRR , CBER, FDA, Silver Spring MD; Department of Bioinformatics and Statistics, NCSU, Raleigh, NC